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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PAK6 Polyclonal antibody

PAK6 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27942-1-AP

Synonyms

p21 activated kinase 6, PAK 5, PAK 6, PAK5, PAK6

Validation Data Gallery

Applications:
  • WB analysis using 27942-1-AP

    WB analysis using 27942-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27942-1-AP (PAK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 27942-1-AP

    WB analysis of mouse brain using 27942-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 27942-1-AP (PAK6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 27942-1-AP

    IHC staining of human testis using 27942-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27942-1-AP (PAK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 27942-1-AP

    IHC staining of human testis using 27942-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27942-1-AP (PAK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

27942-1-AP targets PAK6 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAK6 fusion protein Ag27687 相同性解析による交差性が予測される生物種
Full Name p21 protein (Cdc42/Rac)-activated kinase 6
Calculated molecular weight 681 aa, 75 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC035596
Gene symbol PAK6
Gene ID (NCBI) 56924
RRIDAB_2881015
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAK6(p21-activated kinase 6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. .This protein plays a role in the regulation of gene transcription and may protect cells from apoptosis through phosphorylation of BAD. Northern blot analysis revealed that PAK6 is highly expressed in testis and prostate tissues (PMID:11278661). The immunogen of this antibody is the N-terminal of PAK6. This antibody is specific to PAK6.

Protocols

Product Specific Protocols
WB protocol for PAK6 antibody 27942-1-APDownload protocol
IHC protocol for PAK6 antibody 27942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Pharmacol Res

HPV E7-drived ALKBH5 promotes cervical cancer progression by modulating m6A modification of PAK5

Authors - Fu-Chun Huo
View Article