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  • Featured Product
  • KD/KO Validated

P53 Recombinant antibody

P53 Recombinant Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, zebrafish and More (1)

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2H13

Cat no : 80077-1-RR

Synonyms

Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

Validation Data Gallery

Applications:
  • WB analysis using 80077-1-RR

    WB analysis using 80077-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 80077-1-RR

    WB analysis using 80077-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 80077-1-RR

    WB analysis using 80077-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of zebrafish using 80077-1-RR

    WB analysis of zebrafish using 80077-1-RR

    zebrafish tissue were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 80077-1-RR

    WB analysis of A431 using 80077-1-RR

    WB result of P53 antibody (80077-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P53 transfected A431 cells.

  • WB analysis of ROS1728 using 80077-1-RR

    WB analysis of ROS1728 using 80077-1-RR

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 80077-1-RR

    WB analysis of HSC-T6 using 80077-1-RR

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of ATDC-5 using 80077-1-RR

    WB analysis of ATDC-5 using 80077-1-RR

    ATDC-5 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of Neuro-2a using 80077-1-RR

    WB analysis of Neuro-2a using 80077-1-RR

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 80077-1-RR

    WB analysis using 80077-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 80077-1-RR

    WB analysis of A431 using 80077-1-RR

    A431 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of SW480 using 80077-1-RR

    WB analysis of SW480 using 80077-1-RR

    SW480 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HT-29 using 80077-1-RR

    WB analysis of HT-29 using 80077-1-RR

    HT-29 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 80077-1-RR

    IF Staining of HepG2 using 80077-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using P53 antibody (80077-1-RR, Clone: 2H13 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of A431 using 80077-1-RR

    IF Staining of A431 using 80077-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (80077-1-RR, Clone: 2H13 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • FC experiment of HepG2 using 80077-1-RR

    FC experiment of HepG2 using 80077-1-RR

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human P53 (80077-1-RR, Clone:2H13) (red) labeled with FlexAble CoraLite®488 Antibody Labeling Kit for Rabbit IgG (KFA001), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inMCF-7 cells, COLO 320 cells, SW480 cells, MDA-MB-231 cells, HCT 116 cells, A431 cells, HT-29 cells, ROS1728 cells, HSC-T6 cells, ATDC-5 cells, Neuro-2a cells, zebrafish tissue, HT-29cells
Positive IF detected inHepG2 cells, A431 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

80077-1-RR targets P53 in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat, zebrafish samples.

Tested Reactivity Human, mouse, rat, zebrafish
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name tumor protein p53
Calculated molecular weight 44 kDa
Observed molecular weight53 kDa
GenBank accession numberBC003596
Gene symbol TP53
Gene ID (NCBI) 7157
RRIDAB_2918862
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)

Protocols

Product Specific Protocols
WB protocol for P53 antibody 80077-1-RRDownload protocol
IF protocol for P53 antibody 80077-1-RRDownload protocol
FC protocol for P53 antibody 80077-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Rep

Pseudolaric acid B induces apoptosis associated with the mitochondrial and PI3K/AKT/mTOR pathways in triple‑negative breast cancer

Authors - Ke Yang
View Article
mouseWB,IF

Neurotox Res

NRF2 Antagonizes HIV-1 Tat and Methamphetamine-Induced BV2 Cell Ferroptosis by Regulating SLC7A11

Authors - Shucheng Lin
View Article
humanWB

Acta Biochim Biophys Sin (Shanghai)

Hydrogen sulfide ameliorates senescence in vascular endothelial cells through ameliorating inflammation and activating PPARδ/SGLT2/STAT3 signaling pathway

Authors - Danyang Tian
View Article