CoraLite® Plus 647-conjugated TGF beta 1 Monoclonal antibody

TGF beta 1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1E3E9

Cat no : CL647-69012

Synonyms

CED, DPD1, LAP, TGF ?1, TGF beta 1, TGFB, TGFB1, TGFbeta, TGF-beta1



Tested Applications

Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy TGF beta 1 Ready-To-Use IHC Kit. TGF beta 1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 69012-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL647-69012 targets TGF beta 1 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TGF beta 1 fusion protein HZ-1011 相同性解析による交差性が予測される生物種
Full Name transforming growth factor, beta 1
Gene symbol TGFB1
Gene ID (NCBI) 7040
RRIDAB_2920324
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1).

This antibody is used to Block/Neutralize TGF beta 1 protein.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 TGF beta 1 antibody CL647-69012Download protocol
Standard Protocols
Click here to view our Standard Protocols