• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Neuromedin B Monoclonal antibody

Neuromedin B Monoclonal Antibody for IHC,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1G3B6

Cat no : 66781-1-Ig

Synonyms

neuromedin B, NMB

Validation Data Gallery

Applications:
  • IHC staining of human gliomas using 66781-1-Ig

    IHC staining of human gliomas using 66781-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66781-1-Ig (NMB antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 66781-1-Ig

    IHC staining of human gliomas using 66781-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66781-1-Ig (NMB antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 66781-1-Ig

    IHC staining of mouse brain using 66781-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66781-1-Ig (NMB antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 66781-1-Ig

    IHC staining of mouse brain using 66781-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66781-1-Ig (NMB antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

66781-1-Ig targets Neuromedin B in IHC,ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Neuromedin B fusion protein Ag0842 相同性解析による交差性が予測される生物種
Full Name neuromedin B
Calculated molecular weight 16 kDa
GenBank accession numberBC007407
Gene symbol NMB
Gene ID (NCBI) 4828
RRIDAB_2882126
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neuromedin B (NMB) is a bombesin-like peptide, which inhibits food intake and modulates stress-related behaviour. NMB is encoded in a 76-amino acid precursor. The mature NMB was reported to be a 10-amino-acid peptide, giving two bands of 1.2 kDa and 1.5 kDa in western blotting. NMB is expressed in pituitary gland, hypothalamus, pancreas and stomach. It regulates exocrine and endocrine secretions, cell growth, body temperature and blood pressure and glucose level in vivo. (PMID: 15528253, PMID: 2458345).

Protocols

Product Specific Protocols
IHC protocol for Neuromedin B antibody 66781-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Ann N Y Acad Sci

The von Economo neurons in the frontoinsular and anterior cingulate cortex.

Authors - John M Allman
View Article