CoraLite® Plus 488-conjugated NF-L Monoclonal antibody

NF-L Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5C12G4

Cat no : CL488-60189

Synonyms

68 kDa neurofilament protein, CMT1F, CMT2E, FLJ53642, NEFL, neurofilament, NF, NF L, NF68, NFL, NF-L



Tested Applications

Positive IF detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 60189-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-60189 targets NF-L in IF applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NF-L fusion protein Ag15178 相同性解析による交差性が予測される生物種
Full Name neurofilament, light polypeptide
Calculated molecular weight 543 aa, 62 kDa
GenBank accession numberBC039237
Gene symbol NEFL
Gene ID (NCBI) 4747
RRIDAB_2883115
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NF-L antibody CL488-60189Download protocol
Standard Protocols
Click here to view our Standard Protocols