Napsin A Polyclonal antibody

Napsin A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16558-1-AP

Synonyms

Asp 4, ASP4, Aspartyl protease 4, KAP, Kdap, NAP1, NAPA, NAPSA, Napsin 1, Napsin A, napsin A aspartic peptidase, Pronapsin A, SNAPA, TA01/TA02



Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy Napsin A Ready-To-Use IHC Kit. Napsin A primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

16558-1-AP targets Napsin A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Napsin A fusion protein Ag9786 相同性解析による交差性が予測される生物種
Full Name napsin A aspartic peptidase
Calculated molecular weight 420 aa, 45 kDa
Observed molecular weight 45-55 kDa
GenBank accession numberBC017842
Gene symbol NAPSA
Gene ID (NCBI) 9476
RRIDAB_2878278
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Napsin is found in two isoforms, napsin A and B, with highly homologous nucleotide sequences (91.2%). Napsin A appears to be a functional proteinase, predominantly expressed in lung and kidney. Napsin B is transcribed exclusively in cells related to the immune system and lacks an in-frame stop codon and is believed to be a pseudogene.(PMID:12698189). Napsin A is superior to TTF-1 in distinguishing primary lung ACA from other carcinomas (except kidney), particularly primary lung small cell carcinoma, and primary thyroid carcinoma.(PMID:22288963).

Protocols

Product Specific Protocols
WB protocol for Napsin A antibody 16558-1-APDownload protocol
IHC protocol for Napsin A antibody 16558-1-APDownload protocol
IF protocol for Napsin A antibody 16558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols