CoraLite®594-conjugated Myosin Light Chain 2 Monoclonal antibody

Myosin Light Chain 2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5A1F1

Cat no : CL594-60229

Synonyms

CMH10, DKFZp779C0562, MLC 2, MLC 2v, MLC2, MLC2v, MYL2



Tested Applications

Positive IF detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy MYL2 Ready-To-Use IHC Kit. MYL2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60229-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-60229 is Myosin Light Chain 2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Myosin Light Chain 2 fusion protein Ag1356 相同性解析による交差性が予測される生物種
Full Name myosin, light chain 2, regulatory, cardiac, slow
Calculated molecular weight 19 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC015821
Gene symbol MYL2
Gene ID (NCBI) 4633
RRIDAB_2883447
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.

Protocols

Product Specific Protocols
IF protocol for CL594 Myosin Light Chain 2 antibody CL594-60229Download protocol
Standard Protocols
Click here to view our Standard Protocols