Myosin Light Chain 2/MLC-2V Polyclonal antibody
Myosin Light Chain 2/MLC-2V Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 55462-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | rat heart tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | C2C12 cells |
Planning an IHC experiment? We recommend our IHCeasy MYL2 Ready-To-Use IHC Kit. MYL2 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
55462-1-AP targets Myosin Light Chain 2/MLC-2V in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | myosin, light chain 2, regulatory, cardiac, slow |
| Calculated molecular weight | 19 kDa |
| Observed molecular weight | 18-20 kDa |
| GenBank accession number | NM_000432 |
| Gene symbol | MYL2 |
| Gene ID (NCBI) | 4633 |
| RRID | AB_2881342 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP | Download protocol |
| IHC protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP | Download protocol |
| IP protocol for Myosin Light Chain 2/MLC-2V antibody 55462-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Stem Cell Reports Non-genetic Purification of Ventricular Cardiomyocytes from Differentiating Embryonic Stem Cells through Molecular Beacons Targeting IRX-4. | ||
iScience SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury. | ||
Int J Mol Med Overexpression of CDCA8 promotes the malignant progression of cutaneous melanoma and leads to poor prognosis. |
