CoraLite® Plus 488-conjugated MUM1/IRF4 Polyclonal antibody

MUM1/IRF4 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11247

Synonyms

interferon regulatory factor 4, IRF 4, IRF4, LSIRF, Multiple myeloma oncogene 1, MUM1, MUM1/IRF4, NF EM5



Tested Applications

Positive FC detected inRamos cells
Planning an IHC experiment? We recommend our IHCeasy IRF4 Ready-To-Use IHC Kit. IRF4 primary antibody included.

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-11247 targets MUM1/IRF4 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUM1/IRF4 fusion protein Ag1762 相同性解析による交差性が予測される生物種
Full Name IRF 4
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC015752
Gene symbol IRF4
Gene ID (NCBI) 3662
RRIDAB_2919019
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRF4 is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the ISRE of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 MUM1/IRF4 antibody CL488-11247Download protocol
Standard Protocols
Click here to view our Standard Protocols