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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MTAP Monoclonal antibody

MTAP Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2B1G6

Cat no : 66706-1-Ig

Synonyms

c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase

Validation Data Gallery

Applications:
  • WB analysis of NIH/3T3 using 66706-1-Ig

    WB analysis of NIH/3T3 using 66706-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HCT 116 using 66706-1-Ig

    WB analysis of HCT 116 using 66706-1-Ig

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 66706-1-Ig

    WB analysis of HSC-T6 using 66706-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 66706-1-Ig

    WB analysis of Raji using 66706-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HT-29 using 66706-1-Ig

    WB analysis of HT-29 using 66706-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 66706-1-Ig

    IHC staining of mouse liver using 66706-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 66706-1-Ig

    IHC staining of mouse liver using 66706-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 66706-1-Ig

    IHC staining of mouse kidney using 66706-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 66706-1-Ig

    IHC staining of mouse kidney using 66706-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 66706-1-Ig

    IHC staining of human colon cancer using 66706-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 66706-1-Ig

    IHC staining of human colon cancer using 66706-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 66706-1-Ig

    IHC staining of human colon cancer using 66706-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 66706-1-Ig

    IHC staining of human colon cancer using 66706-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66706-1-Ig

    IHC staining of human liver cancer using 66706-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66706-1-Ig

    IHC staining of human liver cancer using 66706-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66706-1-Ig

    IHC staining of human liver cancer using 66706-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66706-1-Ig

    IHC staining of human liver cancer using 66706-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 66706-1-Ig

    IHC staining of human kidney using 66706-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 66706-1-Ig

    IHC staining of human kidney using 66706-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 66706-1-Ig

    IHC staining of rat liver using 66706-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 66706-1-Ig

    IHC staining of rat liver using 66706-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 66706-1-Ig

    IF Staining of HeLa using 66706-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MTAP antibody (66706-1-Ig, Clone: 2B1G6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inNIH/3T3 cells, HCT 116 cells, HSC-T6 cells, Raji cells, HT-29 cells
Positive IHC detected inmouse liver tissue, human kidney tissue, rat liver tissue, mouse kidney tissue, human colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

66706-1-Ig targets MTAP in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MTAP fusion protein Ag17954 相同性解析による交差性が予測される生物種
Full Name methylthioadenosine phosphorylase
Calculated molecular weight 283 aa, 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC018625
Gene symbol MTAP
Gene ID (NCBI) 4507
RRIDAB_2882058
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.

Protocols

Product Specific Protocols
WB protocol for MTAP antibody 66706-1-IgDownload protocol
IHC protocol for MTAP antibody 66706-1-IgDownload protocol
IF protocol for MTAP antibody 66706-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols