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MME,CD10 Monoclonal antibody

MME,CD10 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

4B9D9

Cat no : 60034-3-Ig

Synonyms

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11



Tested Applications

Positive WB detected inpig kidney tissue, mouse kidney tissue, HEK-293 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human kidney tissue, human tonsil tissue, mouse kidney tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRamos cells, Raji cells
Planning an IHC experiment? We recommend our IHCeasy CD10 Ready-To-Use IHC Kit. CD10 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

60034-3-Ig targets MME,CD10 in WB, IHC, FC, ELISA applications and shows reactivity with human, pig, mouse samples.

Tested Reactivity human, pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MME,CD10 fusion protein Ag0427 相同性解析による交差性が予測される生物種
Full Name membrane metallo-endopeptidase
Calculated molecular weight 750 aa, 86 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC101658
Gene symbol MME
Gene ID (NCBI) 4311
RRIDAB_10641991
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).

Protocols

Product Specific Protocols
WB protocol for MME,CD10 antibody 60034-3-IgDownload protocol
IHC protocol for MME,CD10 antibody 60034-3-IgDownload protocol
FC protocol for MME,CD10 antibody 60034-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols