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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MAP2K4 Monoclonal antibody

MAP2K4 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E4C1

Cat no : 67333-1-Ig

Synonyms

JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1

Validation Data Gallery

Applications:
  • WB analysis using 67333-1-Ig

    WB analysis using 67333-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67333-1-Ig (MAP2K4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67333-1-Ig

    IHC staining of human breast cancer using 67333-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67333-1-Ig (MAP2K4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67333-1-Ig

    IHC staining of human breast cancer using 67333-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67333-1-Ig (MAP2K4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

67333-1-Ig targets MAP2K4 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MAP2K4 fusion protein Ag11348 相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase kinase 4
Calculated molecular weight 399 aa, 44 kDa
Observed molecular weight44-50 kDa
GenBank accession numberBC036032
Gene symbol MAP2K4
Gene ID (NCBI) 6416
RRIDAB_2882591
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEK4 is also named as JNKK1, MAP2K4 , MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MEK4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MAP2K4 antibody 67333-1-IgDownload protocol
IHC protocol for MAP2K4 antibody 67333-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PNAS Nexus

A potent tumor-selective ERK pathway inactivator with high therapeutic index.

Authors - Zehua Zuo
View Article
mouseWB

Phytomedicine

Differences in p38-STAT3-S100A11 signaling after the administration of aristolochic acid I and IVa may account for the disparity in their nephrotoxicity

Authors - Zhong Xian
View Article