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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MALT1 Monoclonal antibody

MALT1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4D3F6

Cat no : 66225-1-Ig

Synonyms

MALT1, MLT, MLT1, Paracaspase

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 66225-1-Ig

    WB analysis of HeLa using 66225-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66225-1-Ig

    WB analysis of HeLa using 66225-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 66225-1-Ig

    WB analysis of Jurkat using 66225-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 66225-1-Ig

    WB analysis of K-562 using 66225-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 66225-1-Ig

    IHC staining of human lymphoma using 66225-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66225-1-Ig (MALT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 66225-1-Ig

    IHC staining of human lymphoma using 66225-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66225-1-Ig (MALT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 66225-1-Ig

    IF Staining of HeLa using 66225-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66225-1-Ig(MALT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

66225-1-Ig targets MALT1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MALT1 fusion protein Ag20632 相同性解析による交差性が予測される生物種
Full Name mucosa associated lymphoid tissue lymphoma translocation gene 1
Calculated molecular weight 824 aa, 92 kDa
Observed molecular weight 92-100 kDa
GenBank accession numberBC030143
Gene symbol MALT1
Gene ID (NCBI) 10892
RRIDAB_2881616
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mucosa-associated lymphoid tissue lymphoma translocation protein 1 belongs to caspase-like protein superfamily.MALT1 plays a potential role in the regulation of apoptosis and induces NFKB activation and required for the normal development of B cells and for the activation of the IKK complex.It acts as a caspase and mediates additional functions related to T cell activation, cleaving both the ubiquitin editing enzyme,TNFAIP3,and the C terminus of BCL10 .It has two isoforms.Researches showed that inhibition of MALT1 blocks A20 and BCL10 cleavage, reduces NFKB activity, and decreases the expression of NFKB targets BCLXL.

Protocols

Product Specific Protocols
WB protocol for MALT1 antibody 66225-1-IgDownload protocol
IHC protocol for MALT1 antibody 66225-1-IgDownload protocol
IF protocol for MALT1 antibody 66225-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Cell Dev Biol

Targeting MALT1 Suppresses the Malignant Progression of Colorectal Cancer via miR-375/miR-365a-3p/NF-κB Axis.

Authors - Rui Qian
View Article