CoraLite® Plus 488-conjugated LYN Monoclonal antibody

LYN Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C7F2

Cat no : CL488-60211

Synonyms

FLJ26625, JTK8, LYN, Tyrosine protein kinase Lyn



Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inRamos cells
Planning an IHC experiment? We recommend our IHCeasy LYN Ready-To-Use IHC Kit. LYN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60211-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-60211 targets LYN in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen LYN fusion protein Ag12723 相同性解析による交差性が予測される生物種
Full Name v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
Calculated molecular weight 512 aa, 59 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC075002
Gene symbol LYN
Gene ID (NCBI) 4067
RRIDAB_2919237
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 LYN antibody CL488-60211Download protocol
FC protocol for CL Plus 488 LYN antibody CL488-60211Download protocol
Standard Protocols
Click here to view our Standard Protocols