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LATS1 Monoclonal antibody

LATS1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A8F1

Cat no : 66569-1-Ig

Synonyms

h warts, LATS1, WARTS, WARTS protein kinase, wts

Validation Data Gallery

Applications:
  • WB analysis using 66569-1-Ig

    WB analysis using 66569-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66569-1-Ig (LATS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 66569-1-Ig

    WB analysis of HepG2 using 66569-1-Ig

    WB result of LATS1 antibody (66569-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-LATS1 transfected HepG2 cells.

  • WB analysis of HepG2 using 66569-1-Ig

    WB analysis of HepG2 using 66569-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66569-1-Ig (LATS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of T-47D using 66569-1-Ig

    WB analysis of T-47D using 66569-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 66569-1-Ig (LATS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66569-1-Ig

    WB analysis of HeLa using 66569-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66569-1-Ig (LATS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66569-1-Ig

    WB analysis of HEK-293 using 66569-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66569-1-Ig (LATS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inT-47D cells, HeLa cells, HEK-293 cells, HepG2 cells, MCF-7 cells, K-562 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

66569-1-Ig targets LATS1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LATS1 fusion protein Ag11140 相同性解析による交差性が予測される生物種
Full Name LATS, large tumor suppressor, homolog 1 (Drosophila)
Calculated molecular weight 15 kDa, 127 kDa
Observed molecular weight 140-150 kDa, 120 kDa
GenBank accession numberBC015665
Gene symbol LATS1
Gene ID (NCBI) 9113
RRIDAB_2881930
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LATS1(Large tumor suppressor homolog 1) is also named as WARTS and belongds to the AGC Ser/Thr protein kinase family. The gene encodes a highly conserved (from fly to human) protein kinase that plays a crucial role in the prevention of tumor formation by controlling the progression of mitosis. The expression of both long(170 kDa) and short lats1 isoforms(120 kDa) in vertebrate retinal cells raises the possibility that these lats1 proteins may act as negative key regulators of the cell cycle each of them performing a unique role (PMID:15777619). In mammalian cells, LATS1 was phosphorylated in a cell cycle-dependent manner and complexed with CDC2 in early mitosis (PMID:9988268). LATS1 also can be detected as 120 kDa and 140-150 kDa, and play a key role in the regulation of Hippo pathway (PMID: 27940445).

Protocols

Product Specific Protocols
WB protocol for LATS1 antibody 66569-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols