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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Cytokeratin 7 Polyclonal antibody

Cytokeratin 7 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 22208-1-AP

Synonyms

Cell and organelle markers, CK 7, CK7, Cytokeratin 7, Cytoskeleton Marker, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

Validation Data Gallery

Applications:
  • WB analysis of A431 using 22208-1-AP

    WB analysis of A431 using 22208-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 22208-1-AP

    WB analysis of HeLa using 22208-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 22208-1-AP

    WB analysis of HepG2 using 22208-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 22208-1-AP

    WB analysis of HepG2 using 22208-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 22208-1-AP

    WB analysis of A431 using 22208-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 22208-1-AP

    IHC staining of human lung cancer using 22208-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human lung cancer using 22208-1-AP

    IHC staining of human lung cancer using 22208-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human pancreas using 22208-1-AP

    IHC staining of human pancreas using 22208-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human pancreas using 22208-1-AP

    IHC staining of human pancreas using 22208-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human breast cancer using 22208-1-AP

    IHC staining of human breast cancer using 22208-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human breast cancer using 22208-1-AP

    IHC staining of human breast cancer using 22208-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human ovary tumor using 22208-1-AP

    IHC staining of human ovary tumor using 22208-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human ovary tumor using 22208-1-AP

    IHC staining of human ovary tumor using 22208-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human kidney using 22208-1-AP

    IHC staining of human kidney using 22208-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22208-1-AP (Cytokeratin 7 antibody at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human kidney using 22208-1-AP

    IHC staining of human kidney using 22208-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22208-1-AP (Cytokeratin 7 antibody at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human lung cancer using 22208-1-AP

    IHC staining of human lung cancer using 22208-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human lung cancer using 22208-1-AP

    IHC staining of human lung cancer using 22208-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human pancreas using 22208-1-AP

    IHC staining of human pancreas using 22208-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IHC staining of human pancreas using 22208-1-AP

    IHC staining of human pancreas using 22208-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22208-1-AP (Cytokeratin 7 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cytokeratin 7 antibody included
  • IF Staining of A549 using 22208-1-AP

    IF Staining of A549 using 22208-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Cytokeratin 7 antibody (22208-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 22208-1-AP

    IF Staining of HeLa using 22208-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytokeratin 7 antibody (22208-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 22208-1-AP

    FC experiment of HeLa using 22208-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.20ug Anti-Human Cytokeratin 7 (22208-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with 90% MeOH.

Tested Applications

Positive WB detected inA431 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman lung cancer tissue, human pancreas tissue, human kidney tissue, human breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells, HeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Cytokeratin 7 Ready-To-Use IHC Kit. Cytokeratin 7 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

22208-1-AP targets Cytokeratin 7 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 7 fusion protein Ag17528 相同性解析による交差性が予測される生物種
Full Name keratin 7
Calculated molecular weight 469 aa, 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC002700
Gene symbol KRT7
Gene ID (NCBI) 3855
RRIDAB_2879030
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 7 antibody 22208-1-APDownload protocol
IHC protocol for Cytokeratin 7 antibody 22208-1-APDownload protocol
IF protocol for Cytokeratin 7 antibody 22208-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Ginekol Pol

Expression profiles of human somatic mesenchymal stem cells derived from fresh endometrium, ectopic-endometrium and umbilical cord

Authors - Chunmei Li
View Article