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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated KRAP/SSFA2 Polyclonal antibody

KRAP/SSFA2 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-14157

Synonyms

Cleavage signal 1 protein, CS 1, CS1, DKFZp313O1039, DKFZp779G0129, FLJ45996, KIAA1927, KRAP, KRAP/SSFA2, SPAG13, sperm specific antigen 2, SSFA2

Validation Data Gallery

Applications:
  • IF Staining of mouse pancreas using CL594-14157

    IF Staining of mouse pancreas using CL594-14157

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite®594 KRAP/SSFA2 antibody (CL594-14157) at dilution of 1:200.

  • IF Staining of mouse pancreas using CL594-14157

    IF Staining of mouse pancreas using CL594-14157

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite®594 KRAP/SSFA2 antibody (CL594-14157) at dilution of 1:200.

Tested Applications

Positive IF detected inmouse pancreas tissue
For other applications, we recommend the unconjugated version of this antibody, 14157-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-14157 targets KRAP/SSFA2 in IF applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KRAP/SSFA2 fusion protein Ag5339 相同性解析による交差性が予測される生物種
Full Name sperm specific antigen 2
Calculated molecular weight 138 kDa
GenBank accession numberBC028706
Gene symbol SSFA2
Gene ID (NCBI) 6744
RRIDAB_2883405
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KRAP (Ki-ras-induced actin-interacting protein), also known as SSFA2, CS1 or SPAG13, is a 1,259 amino acid cytoplasmic protein associated with filamentous-actin (F-actin). KRAP is strongly expressed in pancreas and testis. It is also present in colon cancer cell lines, and the expression is up-regulated by activated K-Ras. KRAP-deficient (KO) mice display decreased adiposity and improved glucose metabolism, implicating KRAP as a potential target for the prevention or treatment of obesity and related diseases. KRAP has also been suggested to be a potential target for cancer. This antibody recognizes endogenous KRAP, which migrates with an apparent molecular mass of 180 kDa in SDS-PAGE. (PMID: 14673706; 21501587; 21873152). This antibody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Protocols

Product Specific Protocols
IF protocol for CL594 KRAP/SSFA2 antibody CL594-14157Download protocol
Standard Protocols
Click here to view our Standard Protocols