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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Integrin Alpha 4 Monoclonal antibody

Integrin Alpha 4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B6A3

Cat no : 67040-1-Ig

Synonyms

CD49D, IA4, Integrin alpha 4, Integrin alpha IV, ITGA4, VLA 4 subunit alpha

Validation Data Gallery

Applications:
  • WB analysis using 67040-1-Ig

    WB analysis using 67040-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67040-1-Ig

    WB analysis using 67040-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of NK-92 using 67040-1-Ig

    WB analysis of NK-92 using 67040-1-Ig

    NK-92 cells were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of U-937 using 67040-1-Ig

    WB analysis of U-937 using 67040-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 67040-1-Ig (Integrin Alpha 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inMOLT-4 cells, Raji cells, U-937 cells, NK-92 cells, THP-1 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

67040-1-Ig targets Integrin Alpha 4 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Integrin Alpha 4 fusion protein Ag28024 相同性解析による交差性が予測される生物種
Full Name integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
Calculated molecular weight 1032 aa, 115 kDa
Observed molecular weight 130-150 kDa
GenBank accession numberBC156712
Gene symbol ITGA4
Gene ID (NCBI) 3676
RRIDAB_2882354
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ITGA4, also named as CD49D, belongs to the integrin alpha chain family. ITGA4 is a receptor for fibronectin, VCAM1 and MADCAM1. ITGA4 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. It recognizes the sequence Q-I-D-S in VCAM1. I It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells ITGA4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.

Protocols

Product Specific Protocols
WB protocol for Integrin Alpha 4 antibody 67040-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Blood Adv

SORT1/LAMP2-mediated Extracellular Vesicle Secretion and Cell Adhesion Are Linked to Lenalidomide Resistance in Multiple Myeloma.

Authors - Tomofumi Yamamoto
View Article