mouse IL-1 beta Polyclonal antibody
IL-1 beta Polyclonal Antibody for FC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse and More (2)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 26048-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | RAW 264.7 cells |
| Positive FC detected in | LPS and Brefeldin A treated RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 62 publications below |
| IHC | See 12 publications below |
| IF | See 6 publications below |
| ELISA | See 1 publications below |
Product Information
26048-1-AP targets IL-1 beta in WB, IHC, IF, FC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IL-1 beta fusion protein Ag23461 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 1 beta |
| Calculated molecular weight | 31 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | NM_008361 |
| Gene symbol | Il1b |
| Gene ID (NCBI) | 16176 |
| RRID | AB_2880351 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-1 beta antibody 26048-1-AP | Download protocol |
| FC protocol for IL-1 beta antibody 26048-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Metabolic reprogramming of proinflammatory macrophages by target delivered roburic acid effectively ameliorates rheumatoid arthritis symptoms | ||
Bioact Mater A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds | ||
ACS Nano Hepatic Stellate Cell- and Liver Microbiome-Specific Delivery System for Dihydrotanshinone I to Ameliorate Liver Fibrosis | ||
Acta Pharm Sin B Ligand-based substituent-anchoring design of selective receptor-interacting protein kinase 1 necroptosis inhibitors for ulcerative colitis therapy | ||
J Control Release Controlled extracellular vesicles release from aminoguanidine nanoparticle-loaded polylysine hydrogel for synergistic treatment of spinal cord injury |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Marina (Verified Customer) (06-29-2021) | I detected pro-il1b from lysate of macrophages using a dilution of 1:1000, and exposing the membrane for 30sec. Really good and specific antibody. I strongly recommend
 |
