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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated ITK Polyclonal antibody

ITK Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21525

Synonyms

EMT, IL2 inducible T cell kinase, ITK, Kinase EMT, LYK, PSCTK2, T cell specific kinase, Tyrosine protein kinase Lyk

Validation Data Gallery

Applications:
  • FC experiment of C57BL/6 mouse splenocytes using CL488-21525

    FC experiment of C57BL/6 mouse splenocytes using CL488-21525

    1X10^6 C57BL/6 mouse splenocytes were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human ITK (CL488-21525), and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2), and 0.2 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • FC experiment of Jurkat using CL488-21525

    FC experiment of Jurkat using CL488-21525

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human ITK (CL488-21525) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of mouse splenocytes using CL488-21525

    FC experiment of mouse splenocytes using CL488-21525

    1X10^6 mouse splenocytes were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human ITK (CL488-21525) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inJurkat cells, C57BL/6 mouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 21525-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-21525 targets ITK in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ITK fusion protein Ag16132 相同性解析による交差性が予測される生物種
Full Name IL2-inducible T-cell kinase
Calculated molecular weight 620 aa, 72 kDa
GenBank accession numberBC109077
Gene symbol ITK
Gene ID (NCBI) 3702
RRIDAB_2919181
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 ITK antibody CL488-21525Download protocol
Standard Protocols
Click here to view our Standard Protocols