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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated IRF1 Polyclonal antibody

IRF1 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11335

Synonyms

interferon regulatory factor 1, IRF 1, IRF1, MAR

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL488-11335

    IF Staining of HepG2 using CL488-11335

    Immunofluorescent analysis of (-20°C Ethanol) fixed IFN gamma treated HepG2 cells using CoraLite® Plus 488 IRF1 antibody (CL488-11335) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-11335

    FC experiment of Jurkat using CL488-11335

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IRF1 (CL488-11335) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inIFN gamma treated HepG2 cells
Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 11335-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-11335 targets IRF1 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRF1 fusion protein Ag1879 相同性解析による交差性が予測される生物種
Full Name IRF 1
Calculated molecular weight 325 aa, 37 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC009483
Gene symbol IRF1
Gene ID (NCBI) 3659
RRIDAB_2919026
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRF1, also named as IFN regulatory factor 1, is a 325 amino acid protein, which belongs to the IRF family. IRF1 as a transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. The calculated molecular weight of IRF1 is 37 kDa, but the modified IRF1 protein is about 45-50 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 IRF1 antibody CL488-11335Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Sci Adv

XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility

Authors - Ming Kuang
View Article