• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-28A Monoclonal antibody

IL-28A Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF

Conjugate

Unconjugated

CloneNo.

3B4A1

Cat no : 60270-1-Ig

Synonyms

Cytokine Zcyto20, IFN lambda 2, IFNL2, IL 28A, IL28A, IL-28A, Interferon lambda 2, Interleukin 28A, ZCYTO20

Validation Data Gallery

Applications:
  • IHC staining of human tonsillitis using 60270-1-Ig

    IHC staining of human tonsillitis using 60270-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60270-1-Ig (IL-28A antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human tonsillitis using 60270-1-Ig

    IHC staining of human tonsillitis using 60270-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60270-1-Ig (IL-28A antibody at dilution of 1:50 (under 40x lens).

  • IHC staining of human small intestine using 60270-1-Ig

    IHC staining of human small intestine using 60270-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 60270-1-Ig (IL-28A antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human small intestine using 60270-1-Ig

    IHC staining of human small intestine using 60270-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 60270-1-Ig (IL-28A antibody at dilution of 1:50 (under 40x lens).

  • IF Staining of human tonsillitis using 60270-1-Ig

    IF Staining of human tonsillitis using 60270-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-28A antibody (60270-1-Ig, Clone: 3B4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 60270-1-Ig

    IF Staining of human tonsillitis using 60270-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-28A antibody (60270-1-Ig, Clone: 3B4A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60270-1-Ig is IL-28A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-28A fusion protein Ag18525 相同性解析による交差性が予測される生物種
Full Name interleukin 28A (interferon, lambda 2)
Calculated molecular weight 200 aa, 22 kDa
GenBank accession numberBC113583
Gene symbol IL28A
Gene ID (NCBI) 282616
RRIDAB_2881390
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL28A, also named as IFNL2 and ZCYT020, is a cytokine with immunomodulatory activity. It up-regulates MHC class I antigen expression. IL28A is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. It plays a significant role in the antiviral immune defense in the intestinal epithelium.

Protocols

Product Specific Protocols
IHC protocol for IL-28A antibody 60270-1-IgDownload protocol
IF protocol for IL-28A antibody 60270-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols