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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated IL-1R2 Monoclonal antibody

IL-1R2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3H4H4

Cat no : CL594-60262

Synonyms

CD121b, CDw121b, IL 1R 2, IL 1R beta, IL 1RT 2, IL 1RT2, IL1R2, IL1RB, Interleukin 1 receptor beta, Interleukin 1 receptor type 2, Interleukin 1 receptor type II

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL594-60262

    IF Staining of HeLa using CL594-60262

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated IL1R2 antibody (CL594-60262, Clone: 3H4H4 ) at dilution of 1:100.

Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60262-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-60262 is IL-1R2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IL-1R2 fusion protein Ag4495 相同性解析による交差性が予測される生物種
Full Name interleukin 1 receptor, type II
Calculated molecular weight 398 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC039031
Gene symbol IL1R2
Gene ID (NCBI) 7850
RRIDAB_2883451
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-1 receptor type 2 (IL1R2, also known as CD121b) is a cytokine receptor that belongs to the interleukin 1 receptor family. It exists in both a membrane bound and soluble form. IL1R2 binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL1R2 serves as a negative regulator of IL-1 signaling, thereby suppressing inflammatory response.

Protocols

Product Specific Protocols
IF protocol for CL594 IL-1R2 antibody CL594-60262Download protocol
Standard Protocols
Click here to view our Standard Protocols