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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-12/IL-23 p40 Monoclonal antibody

IL-12/IL-23 p40 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4B2A8

Cat no : 66288-1-Ig

Synonyms

CLMF, CLMF p40, CLMF2, IL 12 subunit p40, IL 12B, IL12B, IL-12B, Interleukin 12 subunit beta, NKSF, NKSF2

Validation Data Gallery

Applications:
  • WB analysis of human liver using 66288-1-Ig

    WB analysis of human liver using 66288-1-Ig

    human liver tissue were subjected to SDS PAGE followed by western blot with 66288-1-Ig (IL12B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman liver tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66288-1-Ig targets IL-12/IL-23 p40 in WB, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IL-12/IL-23 p40 fusion protein Ag21429 相同性解析による交差性が予測される生物種
Full Name interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
Calculated molecular weight 328 aa, 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC067498
Gene symbol IL12B
Gene ID (NCBI) 3593
RRIDAB_2881671
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

Protocols

Product Specific Protocols
WB protocol for IL-12/IL-23 p40 antibody 66288-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols