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IL-1 Beta Monoclonal antibody

IL-1 Beta Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IP, IHC, IF, FC, Cell treatment, ELISA

Conjugate

Unconjugated

CloneNo.

2A1B4

Cat no : 66737-1-Ig

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1B, IL-1B, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta



Tested Applications

Positive WB detected inA431 cells, U-87 MG cells
Positive IHC detected inhuman lung cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inLPS treated THP-1 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

66737-1-Ig targets IL-1 Beta in WB, IP, IHC, IF, FC, Cell treatment, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human, bovine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-1 Beta fusion protein Ag26030 相同性解析による交差性が予測される生物種
Full Name interleukin 1, beta
Calculated molecular weight 269 aa, 31 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC008678
Gene symbol IL1B
Gene ID (NCBI) 3553
RRIDAB_2882087
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.

Protocols

Product Specific Protocols
WB protocol for IL-1 Beta antibody 66737-1-IgDownload protocol
IHC protocol for IL-1 Beta antibody 66737-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Biomaterials

Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo.

Authors - Angelina D Schoenenberger
IHC

J Nanobiotechnology

Low-dose celecoxib-loaded PCL fibers reverse intervertebral disc degeneration by up-regulating CHSY3 expression

Authors - Yunhao Wang
WB,IHC

Cell Mol Life Sci

circACTA2 inhibits NLRP3 inflammasome-mediated inflammation via interacting with NF-κB in vascular smooth muscle cells

Authors - Yang Bai
WB

Oxid Med Cell Longev

Ellagic Acid Protects Dopamine Neurons via Inhibition of NLRP3 Inflammasome Activation in Microglia.

Authors - Xue-Mei He
WB

Arch Toxicol

SIRT3-dependent mitochondrial redox homeostasis mitigates CHK1 inhibition combined with gemcitabine treatment induced cardiotoxicity in hiPSC-CMs and mice

Authors - Jia-Wen Chen
WB

Aging (Albany NY)

Integrative transcriptomic and metabonomic profiling analyses reveal the molecular mechanism of Chinese traditional medicine huankuile suspension on TNBS-induced ulcerative colitis.

Authors - Zhenglan Han