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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647-conjugated IgD Polyclonal antibody

human IgD Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-16489

Synonyms

FLJ00382, FLJ46727, IgD, IGHD

Validation Data Gallery

Applications:
  • IF Staining of human tonsillitis using CL647-16489

    IF Staining of human tonsillitis using CL647-16489

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®®647 human IgD antibody (CL647-16489) at dilution of 1:200.

Tested Applications

Positive IF detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy human IgD Ready-To-Use IHC Kit. human IgD primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16489-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

CL647-16489 targets human IgD in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen human IgD fusion protein Ag9614 相同性解析による交差性が予測される生物種
Full Name immunoglobulin heavy constant delta
Calculated molecular weight 573 aa, 63 kDa
GenBank accession numberBC021276
Gene symbol IGHD
Gene ID (NCBI) 3495
RRIDAB_2920231
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

IgD is the major antigen receptor isotype on the surface of most peripheral B-cells, where it is coexpressed with IgM. The membrane-bound IgD (mIgD) induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. This antibody recognize the heavy chain (~60 kDa) of IgD.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 human IgD antibody CL647-16489Download protocol
Standard Protocols
Click here to view our Standard Protocols