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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated IFN Gamma Monoclonal antibody

IFN Gamma Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E10G7

Cat no : CL488-69007

Synonyms

IFG, IFI, IFN gamma, IFN γ, IFNG, IFN-gamma, IFNγ, IFN-γ, Immune interferon, Interferon gamma, interferon, gamma, NeutraKine® IFN Gamma

Validation Data Gallery

Applications:
  • FC experiment of human PBMCs using CL488-69007

    FC experiment of human PBMCs using CL488-69007

    1X10^6 PMA+ionomycin+protein transport inhibitors treated for 6h human PBMCs were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human IFN Gamma (CL488-69007, Clone:1E10G7), and 0.25 ug CoraLite® Plus 647 Anti-Human D3' target='_blank'>CD3 (T1' target='_blank'>UCHT1) (CL647-65151, Clone: T1' target='_blank'>UCHT1), and 0.25 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of human PBMCs using CL488-69007

    FC experiment of human PBMCs using CL488-69007

    1X10^6 human PBMCs were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human IFN Gamma (CL488-69007, Clone:1E10G7), and 0.25 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10), and 0.25 ug CoraLite® Plus 647 Anti-Human CD3 (UCHT1) (CL647-65151, Clone: UCHT1). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of Jurkat using CL488-69007

    FC experiment of Jurkat using CL488-69007

    1X10^6 PMA and ionomycin treated Jurkat cells were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human IFN Gamma (CL488-69007, Clone:1E10G7) (red), or 0.25 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of NK92 using CL488-69007

    FC experiment of NK92 using CL488-69007

    1X10^6 NK92 were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human IFN Gamma (CL488-69007, Clone:1E10G7) (red), or 0.25 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inPMA+ionomycin+protein transport inhibitors for 6h human PBMCs, NK92, PMA and ionomycin treated Jurkat cells
For other applications, we recommend the unconjugated version of this antibody, 69007-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-69007 targets IFN Gamma in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IFN Gamma fusion protein HZ-1301 相同性解析による交差性が予測される生物種
Full Name IFN gamma
Gene symbol IFNG
Gene ID (NCBI) 3458
RRIDAB_2919565
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 IFN Gamma antibody CL488-69007Download protocol
Standard Protocols
Click here to view our Standard Protocols