CoraLite® Plus 488-conjugated IDH1 Monoclonal antibody
IDH1 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2A6A2
Cat no : CL488-66197
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy IDH1 Ready-To-Use IHC Kit. IDH1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66197-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-66197 targets IDH1 in IF applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | IDH1 fusion protein Ag19293 相同性解析による交差性が予測される生物種 |
| Full Name | isocitrate dehydrogenase 1 (NADP+), soluble |
| Calculated molecular weight | 414 aa, 47 kDa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | BC012846 |
| Gene symbol | IDH1 |
| Gene ID (NCBI) | 3417 |
| RRID | AB_2883280 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 IDH1 antibody CL488-66197 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
