IDH1 Monoclonal antibody
IDH1 Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2A6A2
Cat no : 66197-1-Ig
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, DU 145 cells, MCF-7 cells |
| Positive IHC detected in | human pancreas tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy IDH1 Ready-To-Use IHC Kit. IDH1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
Product Information
66197-1-Ig targets IDH1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | IDH1 fusion protein Ag19293 相同性解析による交差性が予測される生物種 |
| Full Name | isocitrate dehydrogenase 1 (NADP+), soluble |
| Calculated molecular weight | 414 aa, 47 kDa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | BC012846 |
| Gene symbol | IDH1 |
| Gene ID (NCBI) | 3417 |
| RRID | AB_2881590 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IDH1 antibody 66197-1-Ig | Download protocol |
| IHC protocol for IDH1 antibody 66197-1-Ig | Download protocol |
| IF protocol for IDH1 antibody 66197-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Neurosci TDP-43 extracted from frontotemporal lobar degeneration subject brains displays distinct aggregate assemblies and neurotoxic effects reflecting disease progression rates. | ||
J Immunol Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues |
