CoraLite®594-conjugated HSP60 Monoclonal antibody
HSP60 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF, FC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2F10E7
Cat no : CL594-66041
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy HSPD1 Ready-To-Use IHC Kit. HSPD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66041-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| FC | See 1 publications below |
Product Information
The immunogen of CL594-66041 is HSP60 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | HSP60 fusion protein Ag7848 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock 60kDa protein 1 (chaperonin) |
| Calculated molecular weight | 61 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC003030 |
| Gene symbol | HSPD1 |
| Gene ID (NCBI) | 3329 |
| RRID | AB_2883487 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 HSP60 antibody CL594-66041 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Immunol Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis |
