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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated HSP60 Monoclonal antibody

HSP60 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F10E7

Cat no : CL488-66041

Synonyms

60 kDa chaperonin, Chaperonin 60, CPN60, GROEL, Heat shock protein 60, HLD4, HSP 60, HSP60, HSP65, HSPD1, HuCHA60, P60 lymphocyte protein, SPG13

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL488-66041

    IF Staining of HepG2 using CL488-66041

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66041 (HSP60 antibody) at dilution of 1:50.

  • FC experiment of HepG2 using CL488-66041

    FC experiment of HepG2 using CL488-66041

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human HSP60 (CL488-66041, Clone:2F10E7) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSPD1 Ready-To-Use IHC Kit. HSPD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66041-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-66041 is HSP60 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HSP60 fusion protein Ag7848 相同性解析による交差性が予測される生物種
Full Name heat shock 60kDa protein 1 (chaperonin)
Calculated molecular weight 61 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC003030
Gene symbol HSPD1
Gene ID (NCBI) 3329
RRIDAB_2883235
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSPD1, also known as HSP60, belongs to the chaperonin family and acts as a chaperone to enhance cell survival under physiological stresses. Hsp60 has been shown to be connected with many aspects of cell functions such as protein folding and assembling of polypeptide chains in mitochondria. Recently it has been reported that HSP60 is associated with apoptosis or inhibition of cancer cell growth. (21822415)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 HSP60 antibody CL488-66041Download protocol
FC protocol for CL Plus 488 HSP60 antibody CL488-66041Download protocol
Standard Protocols
Click here to view our Standard Protocols