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HORMAD1 Monoclonal antibody

HORMAD1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D7E12

Cat no : 67091-1-Ig

Synonyms

Cancer/testis antigen 46, CT46, DKFZp434A1315, HORMA domain containing 1, HORMAD1, Newborn ovary HORMA protein, NOHMA, RP11 363I22.1



Tested Applications

Positive WB detected inhuman testis tissue, mouse testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

67091-1-Ig targets HORMAD1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HORMAD1 fusion protein Ag28316 相同性解析による交差性が予測される生物種
Full Name HORMA domain containing 1
Calculated molecular weight 45 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC047406
Gene symbol HORMAD1
Gene ID (NCBI) 84072
RRIDAB_2882396
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HORMA domain-containing proteins regulate interactions between homologous chromosomes (homologs) during meiosis in a wide range of eukaryotes [PMID:21079677]. They also implicated in other processes related to crossover formation, including DSB formation, inhibition of promiscuous formation of the synaptonemal complex (SC), and the meiotic prophase checkpoint that monitors both DSB processing and SCs [PMID:19851446]. HORMAD1 first accumulates on the chromosomes during the leptotene to zygotene stages of meiotic prophase I. As germ cells progress into the pachytene stage, HORMAD1 disappears from the synapsed chromosomal regions. However, once the chromosomes desynapse during the diplotene stage, HORMAD1 again accumulates on the chromosome axis of the desynapsed homologs [PMID:19686734].

Protocols

Product Specific Protocols
WB protocol for HORMAD1 antibody 67091-1-IgDownload protocol
IHC protocol for HORMAD1 antibody 67091-1-IgDownload protocol
IF protocol for HORMAD1 antibody 67091-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Death Discov

The cancer/testis antigen HORMAD1 mediates epithelial-mesenchymal transition to promote tumor growth and metastasis by activating the Wnt/β-catenin signaling pathway in lung cancer.

Authors - Kang Liu
  • KD Validated