HNRNPH1 Monoclonal antibody

HNRNPH1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A6C2

Cat no : 67375-1-Ig

Synonyms

DKFZp686A15170, hnRNP H, hnRNPH, HNRNPH1, HNRPH, HNRPH1



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, U2OS cells, HSC-T6 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

67375-1-Ig targets HNRNPH1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HNRNPH1 fusion protein Ag6603 相同性解析による交差性が予測される生物種
Full Name heterogeneous nuclear ribonucleoprotein H1 (H)
Calculated molecular weight 49 kDa
Observed molecular weight 49-50 kDa
GenBank accession numberBC001348
Gene symbol HNRNPH1
Gene ID (NCBI) 3187
RRIDAB_2882623
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708].

Protocols

Product Specific Protocols
WB protocol for HNRNPH1 antibody 67375-1-IgDownload protocol
IHC protocol for HNRNPH1 antibody 67375-1-IgDownload protocol
IF protocol for HNRNPH1 antibody 67375-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

MS0621, a novel small-molecule modulator of Ewing sarcoma chromatin accessibility, interacts with an RNA-associated macromolecular complex and influences RNA splicing

Authors - Tamara Vital