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CoraLite® Plus 488-conjugated HLA class I ABC Monoclonal antibody
HLA class I ABC Monoclonal Antibody for FC, IF
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig
Applications
IF, FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
5C5B7
Cat no : CL488-66013
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | human tonsillitis tissue, Raji cells |
| Positive FC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-66013 targets HLA class I ABC in IF, FC applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | HLA class I ABC fusion protein Ag7488 相同性解析による交差性が予測される生物種 |
| Full Name | major histocompatibility complex, class I, A |
| Calculated molecular weight | 41 kDa |
| Observed molecular weight | 40-44 kDa |
| GenBank accession number | BC003069 |
| Gene symbol | HLA-A |
| Gene ID (NCBI) | 3105 |
| RRID | AB_2883227 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HLA class I ABC antibody CL488-66013 | Download protocol |
| FC protocol for CL Plus 488 HLA class I ABC antibody CL488-66013 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
