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CoraLite® Plus 488-conjugated HEXIM1 Polyclonal antibody

HEXIM1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15676

Synonyms

Cardiac lineage protein 1, CLP1, EDG1, FLJ13562, HEXIM1, HIS1, MAQ1, Menage a quatre protein 1, Protein HEXIM1



Tested Applications

Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HEXIM1 Ready-To-Use IHC Kit. HEXIM1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15676-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-15676 targets HEXIM1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HEXIM1 fusion protein Ag8144 相同性解析による交差性が予測される生物種
Full Name hexamethylene bis-acetamide inducible 1
Calculated molecular weight 41 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC006460
Gene symbol HEXIM1
Gene ID (NCBI) 10614
RRIDAB_2919122
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

HEXIM1, also named CLP1, EDG1, HIS1 or MAQ1, is best known as the inhibitor of positive transcription elongation factor b, which regulates the transcription elongation of RNA polymerase II and controls 60-70% of mRNA synthesis. HEXIM1 regulates NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Previous studies showed that HEXIM1 interacts with two key p53 regulators, nucleophosmin and HDM2, implying a possible connection between HEXIM1 and the p53 signaling pathway.The mainstream MW of this protein is 70 and 54 kDa. Catalog#15676-1-AP recognises both 70 and 54 kDa band.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 HEXIM1 antibody CL488-15676Download protocol
Standard Protocols
Click here to view our Standard Protocols