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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Glutamine Synthetase Monoclonal antibody

Glutamine Synthetase Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B6G4

Cat no : CL594-66323

Synonyms

GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59

Validation Data Gallery

Applications:
  • IF Staining of mouse brain using CL594-66323

    IF Staining of mouse brain using CL594-66323

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Glutamine Synthetase antibody (CL594-66323, Clone: 1B6G4 ) at dilution of 1:200.

  • IF Staining of mouse brain using CL594-66323

    IF Staining of mouse brain using CL594-66323

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Glutamine Synthetase antibody (CL594-66323, Clone: 1B6G4 ) at dilution of 1:200.

  • IF Staining of mouse brain using CL594-66323

    IF Staining of mouse brain using CL594-66323

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Glutamine Synthetase antibody (CL594-66323-2, Clone: 1B6G4 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-66323

    FC experiment of HepG2 using CL594-66323

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Glutamine Synthetase (CL594-66323, Clone:1B6G4) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inmouse brain tissue
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66323-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-66323 targets Glutamine Synthetase in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Glutamine Synthetase fusion protein Ag6309 相同性解析による交差性が予測される生物種
Full Name glutamate-ammonia ligase (glutamine synthetase)
Calculated molecular weight 374 aa, 42 kDa
GenBank accession numberBC011700
Gene symbol GLUL
Gene ID (NCBI) 2752
RRIDAB_2883558
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protocols

Product Specific Protocols
IF protocol for CL594 Glutamine Synthetase antibody CL594-66323Download protocol
Standard Protocols
Click here to view our Standard Protocols