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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Geminin Polyclonal antibody

Geminin Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10802

Synonyms

Gem, Geminin, GMNN, RP3 369A17.3

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-10802

    IF Staining of HepG2 using CL594-10802

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 Geminin antibody (CL594-10802) at dilution of 1:200.

  • FC experiment of MCF-7 using CL594-10802

    FC experiment of MCF-7 using CL594-10802

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human Geminin (CL594-10802) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 10802-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-10802 targets Geminin in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Geminin fusion protein Ag1076 相同性解析による交差性が予測される生物種
Full Name geminin, DNA replication inhibitor
Calculated molecular weight 24 kDa
Observed molecular weight 28-29 kDa
GenBank accession numberBC005185
Gene symbol GMNN
Gene ID (NCBI) 51053
RRIDAB_2919746
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)

Protocols

Product Specific Protocols
IF protocol for CL594 Geminin antibody CL594-10802Download protocol
FC protocol for CL594 Geminin antibody CL594-10802Download protocol
Standard Protocols
Click here to view our Standard Protocols