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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GTF2F2 Polyclonal antibody

GTF2F2 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 15139-1-AP

Synonyms

ATP dependent helicase GTF2F2, BTF4, GTF2F2, RAP30, TF2F2, TFIIF, TFIIF beta

Validation Data Gallery

Applications:
  • WB analysis of K-562 using 15139-1-AP

    WB analysis of K-562 using 15139-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15139-1-AP (F2' target='_blank'>GTF2F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse spleen using 15139-1-AP

    WB analysis of mouse spleen using 15139-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15139-1-AP (F2' target='_blank'>GTF2F2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of rat spleen using 15139-1-AP

    WB analysis of rat spleen using 15139-1-AP

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 15139-1-AP (F2' target='_blank'>GTF2F2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 15139-1-AP

    WB analysis of HeLa using 15139-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15139-1-AP (F2' target='_blank'>GTF2F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 15139-1-AP

    WB analysis of PC-3 using 15139-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15139-1-AP (F2' target='_blank'>GTF2F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15139-1-AP

    IF Staining of HeLa using 15139-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15139-1-AP (F2' target='_blank'>GTF2F2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inK-562 cells, HeLa cells, mouse spleen tissue, PC-3 cells, rat spleen tissue
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GTF2F2 Ready-To-Use IHC Kit. GTF2F2 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15139-1-AP targets GTF2F2 in WB, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GTF2F2 fusion protein Ag7313 相同性解析による交差性が予測される生物種
Full Name general transcription factor IIF, polypeptide 2, 30kDa
Calculated molecular weight 28 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC001771
Gene symbol GTF2F2
Gene ID (NCBI) 2963
RRIDAB_2114554
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GTF2F2 antibody 15139-1-APDownload protocol
IF protocol for GTF2F2 antibody 15139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols