CoraLite® Plus 488-conjugated GRP78/BIP Monoclonal antibody
GRP78/BIP Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat, mouse
Applications
WB
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1D6F7
Cat no : CL488-66574
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, HSC-T6 cells |
Planning an IHC experiment? We recommend our IHCeasy GRP78/HSPA5 Ready-To-Use IHC Kit. GRP78/HSPA5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66574-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-66574 targets GRP78/BIP in WB applications and shows reactivity with Human, rat, mouse samples.
| Tested Reactivity | Human, rat, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GRP78/BIP fusion protein Ag2188 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) |
| Calculated molecular weight | 78 kDa |
| Observed molecular weight | 78 kDa |
| GenBank accession number | BC020235 |
| Gene symbol | HSPA5 |
| Gene ID (NCBI) | 3309 |
| RRID | AB_2919345 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 GRP78/BIP antibody CL488-66574 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
