- Featured Product
- KD/KO Validated
GRP78,BIP Recombinant antibody
GRP78,BIP Recombinant Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
5F17
Cat no : 80849-1-RR
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, RAW264.7 cells, HSC-T6 cells, pig liver tissue |
| Positive IHC detected in | human colon cancer tissue, human pancreas cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy GRP78/HSPA5 Ready-To-Use IHC Kit. GRP78/HSPA5 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| IF | See 1 publications below |
Product Information
80849-1-RR targets GRP78,BIP in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | pig |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | GRP78,BIP fusion protein Ag2188 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) |
| Calculated molecular weight | 78 kDa |
| Observed molecular weight | 70,78 kDa |
| GenBank accession number | BC020235 |
| Gene symbol | HSPA5 |
| Gene ID (NCBI) | 3309 |
| RRID | AB_2918916 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GRP78,BIP antibody 80849-1-RR | Download protocol |
| IHC protocol for GRP78,BIP antibody 80849-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Agric Food Chem Pterostilbene Prevents Tunicamycin-Induced Intestinal Barrier Damage by Targeting Endoplasmic Reticulum Stress, Oxidative Stress, Autophagy, and Gut Microbiota |
