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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD42a / GP9 Polyclonal antibody

CD42a / GP9 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14564-1-AP

Synonyms

CD42a, Glycoprotein 9, glycoprotein IX (platelet), GP IX, GP9, GPIX, Platelet glycoprotein IX

Validation Data Gallery

Applications:
  • WB analysis of human peripheral blood platelets using 14564-1-AP

    WB analysis of human peripheral blood platelets using 14564-1-AP

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 14564-1-AP (CD42a / GP9 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 14564-1-AP

    IHC staining of human spleen using 14564-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14564-1-AP (CD42a / GP9 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human spleen using 14564-1-AP

    IHC staining of human spleen using 14564-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14564-1-AP (CD42a / GP9 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHuman peripheral blood platelets
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14564-1-AP targets CD42a / GP9 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD42a / GP9 fusion protein Ag6129 相同性解析による交差性が予測される生物種
Full Name glycoprotein IX (platelet)
Calculated molecular weight 19 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC030229
Gene symbol GP9
Gene ID (NCBI) 2815
RRIDAB_2878067
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Platelet glycoprotein IX (GP9, also known as CD42a) is a relatively small surface glycoprotein present on platelets and megakaryocytes. GP9 forms a 1:1 non-covalent complex with GPIb (CD42b). The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. Defects in GP9 are a cause of Bernard-Soulier syndrome, also known as giant platelet disease.

Protocols

Product Specific Protocols
WB protocol for CD42a / GP9 antibody 14564-1-APDownload protocol
IHC protocol for CD42a / GP9 antibody 14564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Cell Death Dis

Essential role of zyxin in platelet biogenesis and glycoprotein Ib-IX surface expression

Authors - Rong Yan
View Article