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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated GOT1 Monoclonal antibody

GOT1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5A12E10

Cat no : CL594-60317

Synonyms

CAT, GIG18, GOT1, Transaminase A

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-60317

    IF Staining of HepG2 using CL594-60317

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 GOT1 antibody (CL594-60317, Clone: 5A12E10 ) at dilution of 1:200.

  • IF Staining of HepG2 using CL594-60317

    IF Staining of HepG2 using CL594-60317

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 GOT1 antibody (CL594-60317, Clone: 5A12E10 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 60317-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-60317 targets GOT1 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GOT1 fusion protein Ag7012 相同性解析による交差性が予測される生物種
Full Name glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
Calculated molecular weight 46 kDa
Observed molecular weight 40-46 kDa
GenBank accession numberBC000498
Gene symbol GOT1
Gene ID (NCBI) 2805
RRIDAB_2883463
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.

Protocols

Product Specific Protocols
IF protocol for CL594 GOT1 antibody CL594-60317Download protocol
Standard Protocols
Click here to view our Standard Protocols