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  • KD/KO Validated

GHITM Polyclonal antibody

GHITM Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16296-1-AP

Synonyms

DERP2, GHITM, HSPC282, MICS1, My021, PTD010, TMBIM5, UNQ244/PRO281



Tested Applications

Positive WB detected inmouse liver tissue, HEK-293 cells, HeLa cells, SH-SY5Y cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16296-1-AP targets GHITM in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GHITM fusion protein Ag9482 相同性解析による交差性が予測される生物種
Full Name growth hormone inducible transmembrane protein
Calculated molecular weight 345 aa, 37 kDa
Observed molecular weight 42 kDa, 25-27 kDa
GenBank accession numberBC010354
Gene symbol GHITM
Gene ID (NCBI) 27069
RRIDAB_2111275
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GHITM, also known as MICS1, TMBIM5 or DERP2, is a mitochondrial protein which localizes in the inner membrane. GHITM is involved in mitochondrial morphology in specific cristae structures and the apoptotic release of cytochrome c from the mitochondria (PMID: 18417609). The gene of GHITM maps to chromosome 10q23.1, and encodes a 345-amino-acid protein with a calculated molecular mass of 37 kDa. The apparent molecular weight has been reported to be 42 kDa, the increased size in the protein may be due to post-translational modifications (PMID: 11416014; 16412389). GHITM can be cleaved into smaller forms of 23-27 kDa (PMID: 16412389; 18417609).

Protocols

Product Specific Protocols
WB protocol for GHITM antibody 16296-1-APDownload protocol
IHC protocol for GHITM antibody 16296-1-APDownload protocol
IF protocol for GHITM antibody 16296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

Regulation of mitochondrial proteostasis by the proton gradient

Authors - Maria Patron
humanWB

Cells

Transmembrane BAX Inhibitor-1 Motif Containing Protein 5 (TMBIM5) Sustains Mitochondrial Structure, Shape, and Function by Impacting the Mitochondrial Protein Synthesis Machinery.

Authors - Bruno Seitaj
  • KO Validated
humanWB

Life Sci Alliance

TMBIM5 loss of function alters mitochondrial matrix ion homeostasis and causes a skeletal myopathy.

Authors - Li Zhang
mouseWB

Nat Commun

Early macrophage response to obesity encompasses Interferon Regulatory Factor 5 regulated mitochondrial architecture remodelling

Authors - L. Orliaguet