CoraLite®594-conjugated GFP tag Polyclonal antibody
GFP tag Polyclonal Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
aequorea victoria, recombinant protein
Applications
IF
Conjugate
CoraLite®594 Fluorescent Dye
Cat no : CL594-50430
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | Transfected HEK-293 cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
CL594-50430 targets GFP tag in IF applications and shows reactivity with aequorea victoria, recombinant protein samples.
Tested Reactivity | aequorea victoria, recombinant protein |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GFP tag fusion protein Ag2128 相同性解析による交差性が予測される生物種 |
Full Name | GFP tag |
Calculated molecular weight | 26 kDa |
GenBank accession number | U73901 |
Gene symbol | |
Gene ID (NCBI) | |
RRID | AB_2919882 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP.
Protocols
Product Specific Protocols | |
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IF protocol for CL594 GFP tag antibody CL594-50430 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |