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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Factor XII Monoclonal antibody

Factor XII Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E2F11

Cat no : CL488-66089

Synonyms

Coagulation factor XII, F12, Factor XII, HAE3, HAEX, HAF, Hageman factor

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL488-66089

    IF Staining of HepG2 using CL488-66089

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66089 (Factor XII antibody) at dilution of 1:100.

  • IF Staining of mouse liver using CL488-66089

    IF Staining of mouse liver using CL488-66089

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 Factor XII antibody (CL488-66089, Clone: 2E2F11 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHepG2 cells, mouse liver tissue
For other applications, we recommend the unconjugated version of this antibody, 66089-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66089 targets Factor XII in IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Factor XII fusion protein Ag3401 相同性解析による交差性が予測される生物種
Full Name coagulation factor XII (Hageman factor)
Calculated molecular weight 615 aa, 68 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC012390
Gene symbol F12
Gene ID (NCBI) 2161
RRIDAB_2883253
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Factor XII (F XII, Hageman factor) is a 80 kDa, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Factor XII antibody CL488-66089Download protocol
Standard Protocols
Click here to view our Standard Protocols