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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FUT3 Monoclonal antibody

FUT3 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A6G7

Cat no : 67344-1-Ig

Synonyms

CD174, FT3B, Fucosyltransferase 3, Fucosyltransferase III, FucT III, FUT3, LE, Les, Lewis FT

Validation Data Gallery

Applications:
  • WB analysis of human placenta using 67344-1-Ig

    WB analysis of human placenta using 67344-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of NCCIT using 67344-1-Ig

    WB analysis of NCCIT using 67344-1-Ig

    NCCIT cells were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of JAR using 67344-1-Ig

    WB analysis of JAR using 67344-1-Ig

    JAR cells were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 67344-1-Ig

    WB analysis of HepG2 using 67344-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of JAR using 67344-1-Ig

    WB analysis of JAR using 67344-1-Ig

    JAR cells were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate hyperplasia using 67344-1-Ig

    IHC staining of human prostate hyperplasia using 67344-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 67344-1-Ig (FUT3 antibody) at dilution of 1:500 (under 10x lens)..

  • IHC staining of human prostate hyperplasia using 67344-1-Ig

    IHC staining of human prostate hyperplasia using 67344-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 67344-1-Ig (FUT3 antibody) at dilution of 1:500 (under 40x lens)..

  • IF Staining of human prostate hyperplasia using 67344-1-Ig

    IF Staining of human prostate hyperplasia using 67344-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate hyperplasia tissue using FUT3 antibody (67344-1-Ig, Clone: 2A6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human prostate hyperplasia using 67344-1-Ig

    IF Staining of human prostate hyperplasia using 67344-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate hyperplasia tissue using FUT3 antibody (67344-1-Ig, Clone: 2A6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman placenta tissue, NCCIT cells, JAR cells, HepG2 cells
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman prostate hyperplasia tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67344-1-Ig targets FUT3 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FUT3 fusion protein Ag12581 相同性解析による交差性が予測される生物種
Full Name fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group)
Calculated molecular weight 361 aa, 42 kDa
Observed molecular weight 42-45 kDa
GenBank accession numberBC108675
Gene symbol FUT3
Gene ID (NCBI) 2525
RRIDAB_2882602
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FUT3 antibody 67344-1-IgDownload protocol
IHC protocol for FUT3 antibody 67344-1-IgDownload protocol
IF protocol for FUT3 antibody 67344-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols