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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FRZB Monoclonal antibody

FRZB Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B4A1

Cat no : 67081-1-Ig

Synonyms

FIZ, FRE, Frezzled, FRITZ, frizzled related protein, Frizzled related protein 1, FRP, FRP 3, FRZB, FRZB 1, FRZB PEN, FRZB1, FZRB, hFIZ, sFRP 3, SFRP3, SRFP3

Validation Data Gallery

Applications:
  • WB analysis using 67081-1-Ig

    WB analysis using 67081-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67081-1-Ig (FRZB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of 4T1 using 67081-1-Ig

    WB analysis of 4T1 using 67081-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67081-1-Ig (FRZB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inLNCaP cells, 4T1 cells, MKN-45 cells, SGC-7901 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

67081-1-Ig targets FRZB in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FRZB fusion protein Ag28147 相同性解析による交差性が予測される生物種
Full Name frizzled-related protein
Calculated molecular weight 325 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC027855
Gene symbol FRZB
Gene ID (NCBI) 2487
RRIDAB_2882389
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Frzb (also known as sFRP3) is a member of the secreted frizzled related protein (SFRP) family, originally identified as a chondrogenic factor during bone morphogenesis (PMID: 29209231). It was subsequently shown to be a regulator in Wnt signaling, and also have a role in regulating cell growth and differentiation in specific cell types. FRZB was seen to be acting as an oncogene in some cancer, such as metastatic renal cancer.

Protocols

Product Specific Protocols
WB protocol for FRZB antibody 67081-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Stem Cells Dev

Chlorogenic Acid Promotes Osteogenic Differentiation of Human Dental Pulp Stem Cells Through Wnt Signaling.

Authors - Xiaoping Hu
View Article