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- KD/KO Validated
FBXW7 Polyclonal antibody
FBXW7 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse and More (3)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 28424-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, MCF-7 cells, mouse brain tissue |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 9 publications below |
| WB | See 26 publications below |
| IHC | See 6 publications below |
| IF | See 3 publications below |
| IP | See 5 publications below |
Product Information
28424-1-AP targets FBXW7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FBXW7 fusion protein Ag28471 相同性解析による交差性が予測される生物種 |
| Full Name | F-box and WD repeat domain containing 7 |
| Calculated molecular weight | 66 kDa |
| Observed molecular weight | 100-110 kDa, 66-75 kDa |
| GenBank accession number | NM_001349798 |
| Gene symbol | FBXW7 |
| Gene ID (NCBI) | 55294 |
| RRID | AB_2881138 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FBXW7, also named as FBW7, FBX30, SEL10 and hAgo, is a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It probably recognizes and binds to phosphorylated target proteins. FBXW7 is involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. FBXW7 is a general tumor suppressor in human cancer (PMID: 17909001). FBXW7 has 3 isoforms (α/β/γ) with the calculated molecular mass of 80 kDa, 70 kDa and 66 kDa, and apparent molecular mass of 100-110 kDa and 66-75 kDa (PMID: 31346036/PMID: 22864569). K48 autoubiquitination is consistent with maintenance of ubiquitinated FBW7 (Ub-FBW7, 200 kDa)(PMID: 32353058).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FBXW7 antibody 28424-1-AP | Download protocol |
| IHC protocol for FBXW7 antibody 28424-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer Loss of FBXW7-mediated degradation of BRAF elicits resistance to BET inhibitors in adult T cell leukemia cells. | ||
Theranostics Histone demethylase JMJD3 protects against renal fibrosis by suppressing TGFβ and Notch signaling and preserving PTEN expression. | ||
EMBO Rep The 18S rRNA m6 A methyltransferase METTL5 promotes mouse embryonic stem cell differentiation. | ||
Int Immunopharmacol MiR-223-3p regulates the eosinophil degranulation and enhances the inflammation in allergic rhinitis by targeting FBXW7 | ||
Br J Cancer TRIP13 promotes the cell proliferation, migration and invasion of glioblastoma through the FBXW7/c-MYC axis.
| ||
Front Cell Dev Biol Autophagy Regulates VDAC3 Ubiquitination by FBXW7 to Promote Erastin-Induced Ferroptosis in Acute Lymphoblastic Leukemia. |
