ERC1 Monoclonal antibody

ERC1 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2A5B9

Cat no : 66681-1-Ig

Synonyms

Cast2, ELKS, ERC 1, ERC1, KIAA1081, Rab6 interacting protein 2, RAB6IP2



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

66681-1-Ig targets ERC1 in WB,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity canine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ERC1 fusion protein Ag17665 相同性解析による交差性が予測される生物種
Full Name ELKS/RAB6-interacting/CAST family member 1
Calculated molecular weight 1116 aa, 128 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC132782
Gene symbol ERC1
Gene ID (NCBI) 23085
RRIDAB_2882035
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ERC is an acronym based on previous separate namings of members of this protein family, ELKS, Rab6IP2 and CAST (PMID: 12391317). They are known to bind RIMs, the active zone proteins that regulate neurotransmitter release. ERC1 (ELKS) is an essential regulatory subunit of the IKK complex and likely functions by recruiting IκBα to the complex (PMID: 15218148). Multiple isoforms of ERC1 mRNA are generated by alternative splicing (PMID: 12203787). There are two known splice variants of ERC1 protein that differ at their C termini, ERC1a and ERC1b. ERC1a is ubiquitously expressed, whereas ERC1b is brain-specific (PMID: 12391317; 12923177).

Protocols

Product Specific Protocols
WB protocol for ERC1 antibody 66681-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
canineWB

Biochem Biophys Res Commun

Knockout analysis of Rab6 effector proteins revealed the role of VPS52 in the secretory pathway.

Authors - Yuta Homma