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Species Reactivity

Species Reactivity

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  • Featured Product
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EPOR Polyclonal antibody

EPOR Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55308-1-AP

Synonyms

EPO R, EPOR, erythropoietin receptor

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 55308-1-AP

    WB analysis of Jurkat using 55308-1-AP

    WB result of EPOR antibody (55308-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPOR transfected Jurkat cells.

  • WB analysis of HepG2 using 55308-1-AP

    WB analysis of HepG2 using 55308-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55308-1-AP (EPOR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, K-562 cells, mouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

55308-1-AP targets EPOR in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name erythropoietin receptor
Calculated molecular weight 55 kDa
Observed molecular weight 66 kDa, 78 kDa
GenBank accession numberNM_000121
Gene symbol EPOR
Gene ID (NCBI) 2057
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Erythropoietin (EPO) receptor (EPOR) is a glycoprotein that belongs to the type I superfamily of single-transmembrane cytokine receptors. It consists of an extracellular domain that binds to the EPO ligand, a transmembrane domain and an intracellular domain. The interaction of EPO and EPOR triggers the activation of several signaling pathways that induce erythropoiesis, including JAK2/STAT5, PI3K/AKT, and MAPK (PMID: 34281163). EPOR is present on erythroid progenitor cells and has also been detected on a wide variety of non-hematopoietic cells (PMID: 36233351). The calculated molecular weight of EPOR is 55 kDa. EPOR undergoes posttranslational modification and the apparent molecular weight of 66-78 kDa has been reported (PMID: 8943308; 12088257).

Protocols

Product Specific Protocols
WB protocol for EPOR antibody 55308-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PeerJ

RNA N6-methyladenosine reader IGF2BP3 promotes acute myeloid leukemia progression by controlling stabilization of EPOR mRNA

Authors - Jin Fan
View Article